Nasocomial infections are considered to be one of the important unsolved problems around the world. A lack of knowledge regarding infection control among nurses decreases compliance with these practices around the world. This descriptive hospital-based study was conducted in Omdurman Military Hospital, It aimed at assessing knowledge of nurses regarding infection control in Intensive Care Unit (ICU). The study covered 30 nurses. They constitute the available sample during the study period2014-2015. Data were collected by a questionnaire designed for the study. Data were analyzed using the Statistical Packages for Social Sciences (SPSS). The results showed that (40%) of the study sample washed their hand after any procedure, (50%) of the study sample did not use gown before touching the body fluid. (6%) washed their hands as soon as they come,(26.7%)washed their hands before going to patients, (43.3%)of respondents wearing gloves when touching body fluid (50%)of respondents used personal protective measures (40%) of the study sample kept equipment and supplies sterile (26,7%)of the respondent use a septic technique when inserting intravascular catheters, (53.3%) of respondents replaced tubing used in blood, blood product within 24 hours to avoid infection and occlusion the sets of fluids. Knowledge about infection control were inadequate. The study concluded that knowledge of intensive care unit nurses staff could be improved by providing them with well-organized practical training. The study recommended that there is providing training programs for the newly joined ICU nurses about infection control precautions at regular intervals is recommended.