Introduction: Although obesity, particularly visceral obesity is associated with insulin resistance (1), the mechanism by which adipose tissue modulates insulin sensitivity is controversial. Increase in body mass index has been reported to increase insulin level and ferritin at the iron stores. Vitamin D has also been found to influence insulin secretion insulin secretion and ferritin at the iron stores. Objectives: Analysis of variance (ANOVA) of vitamin D and body mass index on fasting insulin was done to understand their influence on insulin level. Materials and methods: Four groups were formed including low vitamin D-low body mass index (X1), low vitamin D-high body mass index (X2), high vitamin D- low body mass index (X3) and high vitamin D-high body mass index (X4). In the high vitamin D - high body mass index group there was marked increase in fasting insulin. Calculated F - ratio for Analysis of variance showed a value of 6.48 which was much higher than the table value of 2.76. Mean differences of two ANOVA pairs were found to be significantly different (X1 - X4 and X3 - X4). In this study, the groups X1 and X4 were further analyzed and compared. Insulin and ferritin was found to have a positive correlation in group X4, X1 - X4 and in the total sample (all four groups). Result: The results showed that increase in vitamin D and body mass index increases fasting insulin level and ferritin at the iron stores. The standard deviation of fasting insulin was almost 50% of mean in group X4 and the standard deviation of ferritin was more than the mean ferritin in X4 groups. Conclusion: These results showed that vitamin D and body mass index increases fasting insulin level and ferritin at iron stores.