Aim: To determine the effect of audio-visual stimulus on autonomic nervous system. Materials and Methods: The present prospective comparative study was conducted among patients visited the OPD, Darbhanga Medical College and Hospital, Bihar, India. 30 patients of either sex were included. The participant’s vitals (respiration rate, blood pressure, and heart rate) were measured before and after intervention Results: Mean age of the study population was 32.15 years. Mean heart rate, blood pressure, and respiration rate were found statistically lower in group II as compare to group I (p≤0.05) respectively. Conclusion: The findings of the present study concluded that the positive impacts of music in terms of reducing blood pressure, respiratory rate and heart rates.