The cytotoxicity activity of acetal and hexane extracts of Entada mannii (Fabaceae) were evaluate in vitro. E. mannii is a plant used in the traditional treatment of diabetes and several diseases in the south-east of Coast Ivory. In addition to this antidiabetic activity, this plant rich in polyphenolic compounds has an antioxidant potential that could be beneficial in the management of diseases related to oxidative stress. The determination of mitochondrial synthesis by assaying MTT is the principle of the cytotoxicity assay used in this study which was performed on cells of the L-6 cell line (rat skeletal muscle, myoblast). The IC50 of the acetal (AEEM, IC50 = 141.5115 μg / mL) and hexane extracts (HEEM = 99.2682 μg / mL) determined in this study are much higher than the respective pharmacological doses (antioxidant activity) which are (AEEM, IC50 = = 52.30 ± 2.05 μg / mL) and (HEEM, IC50 = 70.51 ± 1.84 μg / mL). The acetal and hexane extracts of Entada mannii thus offer interesting margins of safety which could be an additional advantage for the use of these extracts.