Sulfur Nanoparticles were prepared by different methods with different size and shapes, when the sulfur present as nanoparticles they have many practical applications in our life. This research work discuss with sulfur nanoparticles synthesis, characterization and applications. With dandruff being a common everyday problem and the market loaded with antidandruff shampoos and such skin care products, it is obvious to assume resourceful research into this area would be both objective to present scenario and lucrative potentially. Nanoparticles are frequently in use in some very powerful antimicrobial, antifungal cosmetics nowadays especially silver. However most metallic nanoparticles are harsh and often toxic, and concern remains on their ill effects. Sulfur, certified as biocompatible to eukaryotes and as soil nutrient it is often used in medicine. Though Sulfur nanoparticles are not much worked with it still is a strong antifungal agent and have been used in macro amount in shampoos. A surfactant has some natural antimicrobial property and therefore such combination could be a potential antidandruff hair washing formulation. To check its antidandruff activity, experiments have been conducted on Malassezia furfur; the causal organism for seborrheaic dermatitis or dandruff, which have been cultured for such study in our lab. Spectroscopy based microbial growth kinetics and colony inhibition studies have been performed to show that nanoparticles of sulfur can reduce the proliferation of Malassezia yeast colonies abundantly, and cause cellular damage which inhibits its growth and viability considerably.