Isolation and identification of Salmonella serovars from clinical samples
This study was carried out to determine the prevalence, distribution, and identification of Salmonella serovars in diarrheagenic in different hospitals of Mahakaushal region. A total of 40 clinical samples were collected from typhoid patient of different hospitals like N. S. C. Bose Govt. Medical Hospital, Govt. Hospital Narsinghpur, Govt. Hospital Katni, Govt. Hospital Sagar, and Govt. H. G. Hospital located in Mahakaushal region. Out of these, only 16 samples showed the presence of Salmonella after screening with selective enrichment technique. In order to detect Salmonella strains, positive differential and selective biochemical tests such as H2S production, gas production from glucose, citrate consumption and carbohydrate fermentation (including manitol, dolsitol, sorbitol, arabinose and glucose) and negative biochemical tests such as urease test, indole production and carbohydrate fermentation (including sucrose and lactose) were used. A total of nine Serovars of Salmonellae S. typhi, S. cholerasius, S. houtnae, S. bongori, S. salmae, S. indica, S. typhimurium, S. arizonae, S. diarizonae were identified. It was observed that Salmonella isolated from stool had developed simultaneous resistance to commonly used antibiotics. This has led to enormous morbidity in Mahakaushal region.