The medicinal plants represents an enormous reservoir of potential microbial compounds that could be useful as an alternative to synthetic microbicides and are being used to develop drugs. They are the most common source of antimicrobial agents are the part and parcel of human society to compete diseases from the dawn of civilization as they have minimum side effects. The present study deals with the Phytochemistry and Antimicrobial activity of Euphorbia hirta L. belongs to the family Euphorbiaceae. It was found that Methanol extract possess good zone of inhibition whereas water showed activity only on higher concentration. The inhibitory activity may be pathogen specific or due to the phytochemical properties of respective plant species and the solvents used for the extraction of secondary metabolites.